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PRMT5 antibody (Internal Region)

PRMT5 Reactivity: Human, Mouse, Rat, Dog, Cow, Pig WB, EIA Host: Goat Polyclonal unconjugated
Catalog No. ABIN401522
  • Target See all PRMT5 Antibodies
    PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
    Binding Specificity
    • 15
    • 10
    • 9
    • 8
    • 8
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 89
    • 36
    • 18
    • 7
    • 6
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    Human, Mouse, Rat, Dog, Cow, Pig
    Host
    • 80
    • 6
    • 2
    • 1
    Goat
    Clonality
    • 71
    • 17
    • 1
    Polyclonal
    Conjugate
    • 48
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PRMT5 antibody is un-conjugated
    Application
    • 65
    • 39
    • 37
    • 14
    • 14
    • 13
    • 13
    • 8
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Sequence
    RACREKDRDP EAQ
    Specificity
    This antibody detects PRMT5 (Internal). It is expected to recognize both isoforms (NP_006100.2, NP_001034708.1).
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse, Rat, Pig, Dog, CowSpecies reactivity (tested):Human
    Purification
    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
    Immunogen
    Peptide from the internal region of the protein sequence according to NP_006100.2, NP_001034708.1. Genename: PRMT5
    Top Product
    Discover our top product PRMT5 Primary Antibody
  • Application Notes
    Peptide ELISA: antibody detection limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 70 kDa band observed in Jurkat lysates (calculated MW of72.0 kDa and 71.0 kDa according to NP_006100.2, NP_001034708.1).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
  • Target
    PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
    Alternative Name
    PRMT5 (PRMT5 Products)
    Synonyms
    HRMT1L5 antibody, IBP72 antibody, JBP1 antibody, SKB1 antibody, SKB1Hs antibody, Jbp1 antibody, Skb1 antibody, si:zc14a17.2 antibody, skb1 antibody, wu:fb95f10 antibody, zgc:110669 antibody, hsl7 antibody, DDBDRAFT_0187422 antibody, DDBDRAFT_0235403 antibody, DDB_0187422 antibody, DDB_0235403 antibody, NV18830 antibody, jbp1 antibody, ibp72 antibody, skb1hs antibody, hrmt1l5 antibody, protein arginine methyltransferase 5 antibody, protein arginine N-methyltransferase 5 antibody, protein arginine methyltransferase 5 L homeolog antibody, putative arginine N-methyltransferase, type II antibody, Skb1 family protein antibody, Protein arginine N-methyltransferase 5 antibody, PRMT5 antibody, Prmt5 antibody, prmt5 antibody, prmt5.L antibody, LOC100115766 antibody, prmt-5 antibody
    Background
    Arginine methylation is an irreversible post translational modification linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins (Histone 2A, 3, 4), IL enhancer binding factor, nuclear factors (NF45, 90, ILF3, Nucleolin, STAT1, Poly(A) binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.Synonyms: 72 kDa ICln-binding protein, HRMT1L5, Histone-arginine N-methyltransferase PRMT5, IBP72, JBP1, Jak-binding protein 1, Protein Arginine N-Methyltransferase 5, SKB1, SKB1Hs
    Gene ID
    10419
    NCBI Accession
    NP_001034708
    UniProt
    O14744
    Pathways
    Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development
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