CLIP1 antibody (C-Term)

Catalog No. ABIN401523

Product Details anti-CLIP1 Antibody

Target: CLIP1 (CAP-GLY Domain Containing Linker Protein 1 (CLIP1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This CLIP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: c-PHSTHHGSRG EERP

Specificity: This antibody detects CLIP1 / Restin at C-term. It is expected to recognize both reported isoforms (NP_002947.1, NP_937883.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, PigSpecies reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the C-Terminus of the protein sequence according to NP_002947.1, NP_937883.1 . Genename: CLIP1

Application Details

Application Notes: Peptide ELISA: 1/8000. Western blot: Approx 160 kDa band observed in Human Breast Cancer lysates (calculatedMW of 160 kDa according to NP_002947.1. Recommended concentration: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: Human Thyroid Gland shows structuredcytoplasm staining in active epithelial cells. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for CLIP1

Target: CLIP1 (CAP-GLY Domain Containing Linker Protein 1 (CLIP1))

Alternative Name: CLIP1 / Restin (CLIP1 Products)

Synonyms: RSN antibody, CLIP1 antibody, clip170 antibody, clip-170 antibody, CLIP antibody, CLIP-170 antibody, CLIP170 antibody, CYLN1 antibody, 1110007I12Rik antibody, 4631429H07Rik antibody, AV017631 antibody, C81039 antibody, Clip50 antibody, Rsn antibody, mKIAA4046 antibody, restin antibody, CAP-Gly domain containing linker protein 1 antibody, CAP-Gly domain-containing linker protein 1 antibody, CAP-Gly domain containing linker protein 1 L homeolog antibody, CAP-GLY domain containing linker protein 1b antibody, CAP-GLY domain containing linker protein 1 antibody, CLIP1 antibody, LOC100075022 antibody, clip1 antibody, clip1.L antibody, clip1b antibody, Clip1 antibody

Background: CLIP170 associates with the growing ends of microtubules, a type of behaviour known as 'plus end tracking' that is exhibited by proteins called +TIPS. It is known to bind to CLASPS and IQGAP1, and is thought to play a role as a positive regulator of microtubule dynamics. CLIP170 is also involved in targeting of the dynactin complex to the ends of growing microtubules, and may be important in mitosis.Synonyms: CAP-Gly domain-containing linker protein 1, CLIP-170, CYLN1, Cytoplasmic linker protein 1, Cytoplasmic linker protein 170 alpha-2, RSN, Reed-Sternberg intermediate filament-associated protein

Gene ID: 6249

NCBI Accession: NP_002947

UniProt: P30622

Pathways: Microtubule Dynamics