NRIP1 antibody (Internal Region)

Catalog No. ABIN401524

Product Details anti-NRIP1 Antibody

Target: NRIP1 (Nuclear Receptor Interacting Protein 1 (NRIP1))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This NRIP1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: c-EKDSPRLTKT NP

Specificity: This antibody detects RIP140 / NRIP1 (Internal).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_003480.2 . Genename: NRIP1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 140 kDa band observed in Human Brain (Cerebellum)lysates (calculated MW of 120 kDa according to NP_003480.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for NRIP1

Target: NRIP1 (Nuclear Receptor Interacting Protein 1 (NRIP1))

Alternative Name: NRIP1 (NRIP1 Products)

Synonyms: MGC68665 antibody, NRIP1 antibody, RIP140 antibody, 6030458L20Rik antibody, 8430438I05Rik antibody, 9630050P12 antibody, AA959574 antibody, AW456757 antibody, nuclear receptor interacting protein 1 S homeolog antibody, nuclear receptor interacting protein 1 antibody, circumsporozoite protein antibody, nrip1.S antibody, NRIP1 antibody, nrip1 antibody, Nrip1 antibody, LOC100627733 antibody

Background: RIP140 is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as nuclear receptor interacting protein 1 (NRIP1), this protein modulates transcriptional activity of the estrogen receptor. Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Receptor Interacting Protein 140 (RIP 140) has been shown to enhance estrogen receptor transcriptional activity through the AF2 activation domain in an agonist dependent manner and has also been implicated to be involved in other steroid receptor pathways, including the retinoid pathway. RIP 140 has recently been shown to interact with peroxisome proliferator-activated receptor-alpha (PPAR alpha) and has been proposed to competitively inhibit PPAR alpha interaction with Steroid Receptor Coactivator-1 (SRC-1).Synonyms: Nuclear factor RIP140, Nuclear receptor-interacting protein 1, Receptor-interacting protein 140

Gene ID: 8204

NCBI Accession: NP_003480

UniProt: P48552

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway