SUMF1 antibody (Internal Region)

Catalog No. ABIN401525

Product Details anti-SUMF1 Antibody

Target: SUMF1 (Sulfatase Modifying Factor 1 (SUMF1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This SUMF1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: c-ETLNPKGPPS GKDR

Specificity: This antibody detects SUMF1 / FGE (Internal).

Cross-Reactivity (Details): Species reactivity (expected):Rat, Dog, CowSpecies reactivity (tested):Human, Mouse

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_877437.2 . Genename: SUMF1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 16000. Western blot: 0.5-2 μg/mL. Approx 37-40 kDa band observed in Mouse Pancreas, Mouse Eyeand Human Kidney lysates (calculated MW of 40.0 kDa according to NP_877437.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for SUMF1

Target: SUMF1 (Sulfatase Modifying Factor 1 (SUMF1))

Alternative Name: SUMF1 / FGE (SUMF1 Products)

Synonyms: AAPA3037 antibody, FGE antibody, AA543204 antibody, AI463102 antibody, AI851573 antibody, sulfatase modifying factor 1 antibody, SUMF1 antibody, Sumf1 antibody

Background: SUMF1 is a sulfatase modifying enzyme that oxidizes a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also called C(alpha)-formylglycine. Defects in SUMF1 are the cause of multiple sulfatase deficiency (MSD). MSD is a clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Inheritance is autosomal recessive.Synonyms: C-alpha-formylglycine-generating enzyme 1, Sulfatase-modifying factor 1

Gene ID: 285362

NCBI Accession: NP_877437

UniProt: Q8NBK3