E2F7 antibody (Internal Region)

Catalog No. ABIN401533

Product Details anti-E2F7 Antibody

Target: E2F7 (E2F Transcription Factor 7 (E2F7))

Binding Specificity: Internal Region

Reactivity: Human, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This E2F7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: c-DLSKQKKFTP ERNP

Specificity: This antibody detects E2F7 Transcription Factor (Internal).

Cross-Reactivity (Details): Species reactivity (expected):Canine, Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of the protein sequence according to NP_976328.2 . Genename: E2F7

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 16000. Western blot: 0.1-0.3 μg/mL. Approx 100 kDa band observed in Human Brain (Cerebellum),Kidney and Testis lysates (calculated MW of 99.0 kDa according to NP_976328.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer

Target Details for E2F7

Target: E2F7 (E2F Transcription Factor 7 (E2F7))

Alternative Name: E2F7 (E2F7 Products)

Synonyms: E2F7 antibody, E2F-7 antibody, fc43h03 antibody, si:dkey-217k21.2 antibody, wu:fc43h03 antibody, MGC154335 antibody, A630014C11Rik antibody, D10Ertd739e antibody, E2F transcription factor 7 antibody, E2F transcription factor 7 L homeolog antibody, E2F7 antibody, e2f7 antibody, e2f7.L antibody, E2f7 antibody

Background: E2F7 is an inhibitor of E2F-dependent transcription. It regulates a subset of E2F-dependent genes whose products are required for normal cell cycle progession as well as mitigate cellular proliferation of mouse embryo fibroblasts.Synonyms: E2F transcription factor 7, E2F-7, FLJ12981, Transcription factor E2F7

Gene ID: 144455

NCBI Accession: NP_976328

UniProt: Q96AV8