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Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer170) antibody

Details for Product No. ABIN401559, Supplier: Log in to see
Antigen
Epitope
pSer170
31
26
26
25
23
17
10
9
7
6
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
205
118
113
11
10
7
4
3
1
Host
Rabbit
208
14
2
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
168
61
60
40
26
25
9
4
2
2
2
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MARCKS around the phosphorylation site of Serine 170 (G-F-Sp-F-K).
Specificity This antibody detects endogenous levels of MARCKS only when phosphorylated at Threonine 170.
Purification Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name MARCKS (MARCKS Antibody Abstract)
Background MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL
Gene ID 4082
NCBI Accession NP_002347
UniProt P29966
Research Area Cardiovascular
Application Notes Western blot: 1/500approx. 1/1000. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer170) antibody (ABIN401559) anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer170) antibody
 image for anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer170) antibody (ABIN401559) anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer170) antibody (Image 2)
Background publications Ramsden: "MARCKS: a case of molecular exaptation?" in: The international journal of biochemistry & cell biology, Vol. 32, Issue 5, pp. 475-9, 2001 (PubMed).

Graff, Young, Johnson et al.: "Phosphorylation-regulated calmodulin binding to a prominent cellular substrate for protein kinase C." in: The Journal of biological chemistry, Vol. 264, Issue 36, pp. 21818-23, 1990 (PubMed).

Thelen, Rosen, Nairn et al.: "Regulation by phosphorylation of reversible association of a myristoylated protein kinase C substrate with the plasma membrane." in: Nature, Vol. 351, Issue 6324, pp. 320-2, 1991 (PubMed).

Hartwig, Thelen, Rosen et al.: "MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin." in: Nature, Vol. 356, Issue 6370, pp. 618-22, 1992 (PubMed).