EIF4B antibody (AA 420-424)

Catalog No. ABIN401568

Product Details anti-EIF4B Antibody

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Binding Specificity: AA 420-424

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of total eIF4B protein.

Purification: Immunoaffinity Chromatography using epitope-specific peptide.

Immunogen: Peptide sequence around amino acids 420~424 (T-G-S-E-S) derived from Human eIF4B.

Application Details

Application Notes: Western Blot: 1/500approx. 1/1000. Incubate membrane with diluted antibody in 5 % nonfat milk, 1X TBS, 0.1 % Tween-20 at 4 °Cwith gentle shaking, overnight. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for EIF4B

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Alternative Name: EIF4B (EIF4B Products)

Synonyms: EIF-4B antibody, PRO1843 antibody, CG10837 antibody, Dm-eIF4B antibody, Dmel\\CG10837 antibody, Eif4B antibody, eIF-4B-L antibody, eIF-4B-S antibody, eIF-4b antibody, eIF4B antibody, eIF4B-L antibody, eIF4B-S antibody, eIf4b antibody, 2310046H11Rik antibody, AL024095 antibody, C85189 antibody, Eif4a2 antibody, eif4b antibody, fb15c09 antibody, wu:fb15c09 antibody, zgc:101532 antibody, MGC89384 antibody, eukaryotic translation initiation factor 4B antibody, eukaryotic initiation factor 4B antibody, Eukaryotic initiation factor 4B antibody, eukaryotic translation initiation factor 4Bb antibody, eukaryotic translation initiation factor 4Ba antibody, eukaryotic translation initiation factor 4B S homeolog antibody, EIF4B antibody, Eif4B antibody, eIF-4B antibody, Eif4b antibody, eif4bb antibody, eif4b antibody, eif4ba antibody, eif4b.S antibody, LOC100284982 antibody, LOC100380867 antibody

Background: EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).Synonyms: Eukaryotic translation initiation factor 4B, eIF-4B

Gene ID: 1975

NCBI Accession: NP_001408

UniProt: P23588