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CDK6 antibody (Cyclin-Dependent Kinase 6) (pTyr13)

Details for Product anti-CDK6 Antibody No. ABIN401596, Supplier: Log in to see
Antigen
  • 5830411I20
  • AI504062
  • CDK6
  • Crk2
  • PLSTIRE
Alternatives
anti-Human CDK6 antibody for Immunohistochemistry (Frozen Sections)
Epitope
pTyr13
30
27
24
17
8
7
6
5
5
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
236
101
69
3
3
1
Host
Rabbit
163
76
Clonality
Polyclonal
Conjugate
This CDK6 antibody is un-conjugated
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
164
102
80
52
43
33
23
21
21
2
2
2
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from Human CDK6 around the phosphorylation site of Tyrosine 13 (Q-Q-Yp-E-C).
Specificity This Antibody detects endogenous levels of CDK6 only when phosphorylated at Tyrosine 13.
Purification Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name CDK6 (CDK6 Antibody Abstract)
Background CDK6 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of Cdk6 first appears in mid-G1 phase, and is controlled by regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.Synonyms: Cell division protein kinase 6, PLSTIRE, Serine/threonine-protein kinase PLSTIRE
Gene ID 1021
NCBI Accession NP_001250
UniProt Q00534
Research Area Cell Cycle
Pathways Cell Division Cycle
Application Notes Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-CDK6 antibody (Cyclin-Dependent Kinase 6) (pTyr13) (ABIN401596) anti-Cyclin-Dependent Kinase 6 (CDK6) (pTyr13) antibody
 image for anti-CDK6 antibody (Cyclin-Dependent Kinase 6) (pTyr13) (ABIN401596) anti-Cyclin-Dependent Kinase 6 (CDK6) (pTyr13) antibody (Image 2)
 image for anti-CDK6 antibody (Cyclin-Dependent Kinase 6) (pTyr13) (ABIN401596) anti-Cyclin-Dependent Kinase 6 (CDK6) (pTyr13) antibody (Image 3)
Background publications Lukas, Bartkova, Bartek: "Convergence of mitogenic signalling cascades from diverse classes of receptors at the cyclin D-cyclin-dependent kinase-pRb-controlled G1 checkpoint." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6917-25, 1997 (PubMed).

Hirai, Roussel, Kato, Ashmun, Sherr: "Novel INK4 proteins, p19 and p18, are specific inhibitors of the cyclin D-dependent kinases CDK4 and CDK6." in: Molecular and cellular biology, Vol. 15, Issue 5, pp. 2672-81, 1995 (PubMed).

Flores-Rozas, Kelman, Dean, Pan, Harper, Elledge, ODonnell, Hurwitz: "Cdk-interacting protein 1 directly binds with proliferating cell nuclear antigen and inhibits DNA replication catalyzed by the DNA polymerase delta holoenzyme." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 18, pp. 8655-9, 1994 (PubMed).