Perilipin 5 (PLIN5) (AA 451-463), (C-Term) antibody

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Antigen
  • LSDA5
  • LSDP5
  • MLDP
  • OXPAT
  • 2310076L09Rik
  • AI415325
  • AW109675
  • Lsdp5
  • PAT-1
  • lipid storage droplet protein 5
  • perilipin 5
  • LSDP5
  • Plin5
  • PLIN5
Epitope
C-Term, AA 451-463
20
19
17
2
2
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine)
42
39
20
1
Host
Guinea Pig
38
4
1
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
4
4
2
2
2
2
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
42
13
12
11
2
2
2
2
1
Options
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Immunogen Synthetic peptide corresponding to the C-terminal (aa 451-463) of Human MLDP
Sequence CPVKHTLMPE LDF
Specificity This antibody reacts with MLDP = myocardial lipid droplet protein (also described as OXPAT/PAT-1), MW 50,844 (calculated from aa sequence data), Apparent Mr 52,000 (after SDS-PAGE). MLDP pertains to the PAT-family proteins, covering the surface of cytoplasmic lipid droplets and sharing a homologuous domain called PAT. Additional PAT proteins include adipophilin (ADRP), perilipin, and TIP47 which are expressed in differentiation-related stages of lipid metabolism. Tissue Immunolocalization: MLDP is positively detected in heart, skeletal muscle. Tested Reactivities on Cultured Cell Lines: HaCat (human), SV80 (human), 3T3-L1 (mouse).
Cross-Reactivity (Details) Species reactivity (tested):Human, Bovine, Mouse, Rat.
Purification Serum
Alternative Name Perilipin-5 (PLIN5 Antibody Abstract)
Background Synonyms: LSDP5, Lipid storage droplet protein 5, OXPAT, PLIN5
Gene ID 440503
NCBI Accession NP_001013728
UniProt Q00G26
Application Notes Western Blot: 1/2000. Immunohistochemistry on frozen sections: 1/100 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Wolins, Quaynor, Skinner, Tzekov, Croce, Gropler, Varma, Yao-Borengasser, Rasouli, Kern, Finck, Bickel: "OXPAT/PAT-1 is a PPAR-induced lipid droplet protein that promotes fatty acid utilization." in: Diabetes, Vol. 55, Issue 12, pp. 3418-28, 2006 (PubMed).

Wolins, Brasaemle, Bickel: "A proposed model of fat packaging by exchangeable lipid droplet proteins." in: FEBS letters, Vol. 580, Issue 23, pp. 5484-91, 2006 (PubMed).

Garcia-Carbonero, Supko, Maki, Manola, Ryan, Harmon, Puchalski, Goss, Seiden, Waxman, Quigley, Lopez, Sancho, Jimeno, Guzman, Demetri: "Ecteinascidin-743 (ET-743) for chemotherapy-naive patients with advanced soft tissue sarcomas: multicenter phase II and pharmacokinetic study." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 23, Issue 24, pp. 5484-92, 2005 (PubMed).