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ID4 antibody (Middle Region)

ID4 Reactivity: Human, Mouse, Rat, Pig, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN404793
  • Target See all ID4 Antibodies
    ID4 (Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4))
    Binding Specificity
    • 15
    • 14
    • 13
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region
    Reactivity
    • 63
    • 31
    • 19
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Pig, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit
    Host
    • 57
    • 6
    Rabbit
    Clonality
    • 60
    • 3
    Polyclonal
    Conjugate
    • 28
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This ID4 antibody is un-conjugated
    Application
    • 35
    • 29
    • 19
    • 13
    • 13
    • 12
    • 7
    • 3
    • 2
    • 1
    Western Blotting (WB)
    Sequence
    CYSRLRRLVP TIPPNKKVSK VEILQHVIDY ILDLQLALET HPALLRQPPP
    Predicted Reactivity
    Cow: 85%, Dog: 100%, Guinea Pig: 85%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 79%, Rat: 100%, Zebrafish: 86%
    Characteristics
    This is a rabbit polyclonal antibody against ID4. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the middle region of human ID4
    Top Product
    Discover our top product ID4 Primary Antibody
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 161 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    ID4 (Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4))
    Alternative Name
    ID4 (ID4 Products)
    Synonyms
    Idb4 antibody, bHLHb27 antibody, cb547 antibody, fb77d04 antibody, sb:cb547 antibody, wu:fb77d04 antibody, zgc:123214 antibody, bhlhb27 antibody, id4 antibody, id4a antibody, idb4 antibody, IDB4 antibody, inhibitor of DNA binding 4 antibody, inhibitor of DNA binding 4, HLH protein antibody, inhibitor of DNA binding 4, HLH protein S homeolog antibody, Id4 antibody, id4 antibody, id4.S antibody, ID4 antibody
    Background
    Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins.Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 [PubMed 7665172]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
    Alias Symbols: IDB4, bHLHb27
    Protein Interaction Partner: TCF3, TCF4, ID3, MYOD1, HES1,
    Protein Size: 161
    Molecular Weight
    16 kDa
    Gene ID
    3400
    NCBI Accession
    NM_001546, NP_001537
    UniProt
    P47928
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