anti-Nucleolar Protein 6 antibody (NOL6) (N-Term)

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Antigen
Synonyms NRAP, UTP22, bA311H10.1, nrap, utp22, AA410091, Nrap
Epitope
N-Term
(4), (2), (2), (2), (2), (1), (1), (1)
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(19), (5), (5), (1)
Host
Rabbit
(12), (5), (2)
Clonality
Polyclonal
Application
Western Blotting (WB)
(17), (7), (5), (4), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human NOL6
Sequence SRKRTLAEPP AKGLLQPVKL SRAELYKEPT NEELNRLRET EILFHSSLLR
Predicted Reactivity Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, Bovine : 90 %, Rabbit : 90 %, African clawed frog : 70 %, Chicken : 70 %
Characteristics This is a rabbit polyclonal antibody against NOL6. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name NOL6 (NOL6 Antibody Abstract)
Background NOL6 is a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified.
Molecular Weight 127 kDa
Gene ID 65083
NCBI Accession NP_075068, NM_022917
UniProt Q9H6R4
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1146 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Nucleolar Protein 6 antibody (NOL6) (N-Term) (ABIN405371) WB Suggested Anti-NOL6 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Background publications Nousiainen, Silljé, Sauer et al.: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).

Catalog No. ABIN405371
$289.00
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