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VPREB1 antibody (Middle Region)

VPREB1 Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN405461
  • Target See all VPREB1 Antibodies
    VPREB1 (Pre-B Lymphocyte 1 (VPREB1))
    Binding Specificity
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region
    Reactivity
    • 22
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit
    Host
    • 25
    • 1
    Rabbit
    Clonality
    • 25
    • 1
    Polyclonal
    Conjugate
    • 14
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This VPREB1 antibody is un-conjugated
    Application
    • 18
    • 10
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Sequence
    TIRLTCTLRN DHDIGVYSVY WYQQRPGHPP RFLLRYFSQS DKSQGPQVPP
    Predicted Reactivity
    Cow: 92%, Dog: 86%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 79%
    Characteristics
    This is a rabbit polyclonal antibody against VPREB1. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the middle region of human VPREB1
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    Discover our top product VPREB1 Primary Antibody
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 145 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    VPREB1 (Pre-B Lymphocyte 1 (VPREB1))
    Alternative Name
    VPREB1 (VPREB1 Products)
    Synonyms
    IGI antibody, IGVPB antibody, VPREB antibody, VpreB antibody, CD179a antibody, Vpreb-1 antibody, V-set pre-B cell surrogate light chain 1 antibody, immunoglobulin iota chain antibody, immunoglobulin iota chain-like antibody, pre-B lymphocyte 1 antibody, pre-B lymphocyte gene 1 antibody, VPREB1 antibody, LOC698810 antibody, LOC486411 antibody, Vpreb1 antibody
    Background
    VPREB1 belongs to the immunoglobulin superfamily and is expressed selectively at the early stages of B cell development, namely, in proB and early preB cells. This gene encodes the iota polypeptide chain that is associated with the Ig-mu chain to form a molecular complex which is expressed on the surface of pre-B cells. The complex is thought to regulate Ig gene rearrangements in the early steps of B-cell differentiation.CD179a (VpreB) is a 126 aa-long polypeptide with apparent MW of 16-18 kDa. It is expressed selectively at the early stages of B cell development, namely, in proB and early preB cells. CD179a has an Ig V domain-like structure, but lacks the last beta-strand (beta7) of a typical V domain. Instead, it has a carboxyl terminal end that shows no sequence homologies to any other proteins. CD179a associates non-covalently with CD179b (lambda5 or lambda-like) carrying an Ig C domain-like structure to form an Ig light chain-like structure, which is called the surrogate light chain or pseudo light chain. In this complex, the incomplete V domain of CD179a appears to be complemented by the extra beta7 strand of CD179b. On the surface of early preB cells, CD179a/CD179b surrogate light chain is disulfide-linked to membrane-bound Ig mu heavy chain in association with a signal transducer CD79a/CD79b heterodimer to form a B cell receptor-like structure, so-called preB cell receptor (preBCR). Though no CD179a-related human disease or pathology has been reported yet, the deficiency of other components of preB cell receptor such as CD179b, Ig mu heavy chain and CD79a has been shown to result in severe impairment of B cell development and agammaglobulinemia in human. PreBCR transduces signals for: 1) cellular proliferation, differentiation from the proB cell to preB cell stage, 2) allelic exclusion at the Ig heavy chain gene locus, and 3) promotion of Ig light chain gene rearrangements. Thus, preBCR functions as a checkpoint in early B cell development to monitor the production of Ig mu heavy chain through a functional rearrangement of Ig heavy chain gene as well as the potency of Ig mu heavy chain to associate with Ig light chain. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
    Alias Symbols: IGI, IGVPB, VPREB
    Protein Interaction Partner: VPREB3, LGALS1, IGHM,
    Protein Size: 145
    Molecular Weight
    16 kDa
    Gene ID
    7441
    NCBI Accession
    NM_007128, NP_009059
    UniProt
    P12018
    Pathways
    Production of Molecular Mediator of Immune Response, Regulation of Cell Size
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