HECT Domain and Ankyrin Repeat Containing, E3 Ubiquitin Protein Ligase 1 (HACE1) (Middle Region) antibody

Details for Product No. ABIN405544
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Antigen
Synonyms HACE1, 1700042J16Rik, A730034A22Rik, BC025474
Epitope
Middle Region
(2), (2), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Pig (Porcine), Dog (Canine), Xenopus laevis, Human, Chicken
(24), (15), (12), (12), (12), (12), (12), (3)
Host
Rabbit
(19), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(15), (10), (9), (6), (4), (3), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN405544
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Immunogen Synthetic peptide directed towards the middle region of human HACE1
Sequence DVSDWIKNTE YTSGYEREDP VIQWFWEVVE DITQEERVLL LQFVTGSSRV
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, African clawed frog : 80 %, Chicken : 80 %
Characteristics This is a rabbit polyclonal antibody against HACE1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name HACE1
Background HACE1 contains 6 ANK repeats and 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. HACE1 is an E3 ubiquitin-protein ligase that may function in cellular proteins degradation.
Molecular Weight 102 kDa
Gene ID 57531
NCBI Accession NP_065822, NM_020771
UniProt Q8IYU2
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 909 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-HECT Domain and Ankyrin Repeat Containing, E3 Ubiquitin Protein Ligase 1 (HACE1) (Middle Region) antibody WB Suggested Anti-HACE1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Hela cell lysate
anti-HECT Domain and Ankyrin Repeat Containing, E3 Ubiquitin Protein Ligase 1 (HACE1) (Middle Region) antibody (2) Host: Rabbit
Target Name: HACE1
Sample Tissue: Hela Whole cell
Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1 µg/mL Peptide Concentration: 5 µg/mL Lysate Quantity: 41 µg/lane/LaneGel Concentration:.12
Background publications Zhang, Anglesio, OSullivan et al.: "The E3 ligase HACE1 is a critical chromosome 6q21 tumor suppressor involved in multiple cancers." in: Nature medicine, Vol. 13, Issue 9, pp. 1060-9, 2007 (PubMed).

Validation Images
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