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ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b) (Middle Region) antibody

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Antigen
Synonyms ATP6B, AV080843, S76401S1
Epitope
Middle Region
(15), (15), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(70), (27), (25), (2), (2), (1)
Host
Rabbit
(63), (11)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(54), (36), (16), (11), (4), (4), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the middle region of human ATP4B
Blocking Peptide Blocking peptide for this product available: ABIN973613
Sequence QVKYYPPNGT FSLHYFPYYG KKAQPHYSNP LVAAKLLNIP RNAEVAIVCK
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Chicken : 90 %
Characteristics This is a rabbit polyclonal antibody against ATP4B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name ATP4B (ATP4b Antibody Abstract)
Background ATP4B belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.
Molecular Weight 33 kDa
Gene ID 496
NCBI Accession NP_000696, NM_000705
UniProt P51164
Research Area Enzymes, Metabolism
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 291 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b) (Middle Region) antibody (ABIN405642) anti-ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b) (Middle Region) antibody
Background publications Oh, Yang, Hahn et al.: "Transcriptome analysis of human gastric cancer." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 16, Issue 12, pp. 942-54, 2005 (PubMed).

Catalog No. ABIN405642
$289.00
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