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Podoplanin (PDPN) (Middle Region) antibody

Details for Product No. ABIN405667
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Antigen
Synonyms PDPN, AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2, OTS-8, RANDAM-2, T1-alpha, T1a, T1alpha, E11, RTI40
Epitope
Middle Region
(22), (10), (6), (5), (5), (1), (1), (1)
Reactivity
Human
(117), (45), (20), (1)
Host
Rabbit
(80), (51), (13), (12), (5), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application
Western Blotting (WB)
(97), (46), (38), (37), (34), (25), (24), (23), (10), (9), (1), (1), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN405667
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Immunogen Synthetic peptide directed towards the middle region of human PDPN
Sequence VATSHSTEKVDGDTQTTVEKDGLSTVTLVGIIVGVLLAIG FIGAIIVVVM
Predicted Reactivity Human : 100 %
Characteristics This is a rabbit polyclonal antibody against PDPN. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name PDPN
Background PDPN is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified.This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified.
Molecular Weight 25 kDa
Gene ID 10630
NCBI Accession NM_006474, NP_006465
UniProt Q86YL7-3
Research Area Cancer
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 238 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Podoplanin (PDPN) (Middle Region) antibody WB Suggested Anti-PDPN Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Placenta
Background publications Christou, Pearce, Watson et al.: "Renal cells activate the platelet receptor CLEC-2 through podoplanin." in: The Biochemical journal, Vol. 411, Issue 1, pp. 133-40, 2008 (PubMed).

Validation Images
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