DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (Middle Region) antibody

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Synonyms NV11307, DOT1L, DOT1, KMT4, A630076O07, AW907654, Dot1, mDot1
Middle Region
(14), (11), (10), (7), (6), (4), (3), (3), (3), (2), (1), (1)
Human, Rat (Rattus)
(62), (6), (5)
(32), (30)
(3), (3), (2), (2), (2), (2), (1)
Western Blotting (WB)
(34), (23), (16), (8), (6), (5), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
Shipping to United States ( )
Availability Will be delivered in 2 to 3 Business Days
Blocking Peptide Blocking peptide for this product available: Disrupted in Schizophrenia 1 (DISC1) peptide (ABIN975803)
Predicted Reactivity Human :100 %,   Rat :80 %,  
Characteristics This is a rabbit polyclonal antibody against DOT1L. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name DOT1L (DOT1L Antibody Abstract)
Background DOT1L is a histone methyltransferase. It methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones.
Molecular Weight 165 kDa
Gene ID 27185
NCBI Accession NP_115871, NM_032482
UniProt Q8TEK3
Research Area Neurology, Alzheimer's Disease
Application Notes Optimal working dilutions should be determined experimentally by the investigator.

Antigen size: 1537 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (Middle Region) antibody (ABIN405948) WB Suggested Anti-DISC1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Background publications Steger, Lefterova, Ying et al.: "DOT1L/KMT4 recruitment and H3K79 methylation are ubiquitously coupled with gene transcription in mammalian cells." in: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2825-39, 2008 (PubMed).

Catalog No. ABIN405948
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