DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (Middle Region) antibody

Details for Product No. ABIN405948
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Antigen
Synonyms NV11307, DOT1L, DOT1, KMT4, A630076O07, AW907654, Dot1, mDot1
Epitope
Middle Region
(9), (6), (6), (3), (3), (1), (1), (1)
Reactivity
Rat (Rattus), Human
(26)
Host
Rabbit
(19), (6), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(18), (13), (8), (5), (4), (3), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN405948
289.00 $
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Predicted Reactivity Human :100 %,   Rat :80 %,  
Characteristics This is a rabbit polyclonal antibody against DOT1L. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name DOT1L
Background DOT1L is a histone methyltransferase. It methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones.
Molecular Weight 165 kDa
Gene ID 27185
NCBI Accession NP_115871, NM_032482
UniProt Q8TEK3
Research Area Neurology, Alzheimer's Disease
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1537 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (Middle Region) antibody WB Suggested Anti-DISC1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Hela cell lysate
Background publications Steger, Lefterova, Ying et al.: "DOT1L/KMT4 recruitment and H3K79 methylation are ubiquitously coupled with gene transcription in mammalian cells." in: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2825-39, 2008 (PubMed).

Validation Images
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