SET and MYND Domain Containing 3 (SMYD3) (N-Term) antibody

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Antigen
Synonyms KMT3E, ZMYND1, ZNFN3A1, bA74P14.1, 2410008A19Rik, Zmynd1
Epitope
N-Term
(7), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(50), (25), (25), (3), (3), (2)
Host
Rabbit
(48), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
(35), (11), (11), (11), (9), (4), (3), (3), (2), (2), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human SMYD3
Blocking Peptide Blocking peptide for this product available: SET and MYND Domain Containing 3 (SMYD3) (N-Term) peptide (ABIN984157)
Sequence PRYPPDSVRL LGRVVFKLMD GAPSESEKLY SFYDLESNIN KLTEDKKEGL
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against SMYD3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name SMYD3 (SMYD3 Antibody Abstract)
Background SMYD3 is a member of an RNA polymerase complex. Within the RNA polymerase complex, it acts as a histone methyltransferase that plays a role in transcriptional regulation.SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.
Molecular Weight 42 kDa
Gene ID 64754
NCBI Accession NP_073580, NM_022743
Research Area Cancer, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 369 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-SET and MYND Domain Containing 3 (SMYD3) (N-Term) antibody (ABIN406136) WB Suggested Anti-SMYD3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Chromatin Immunoprecipitation (ChiP) image for anti-SET and MYND Domain Containing 3 (SMYD3) (N-Term) antibody (ABIN406136) Quiescent human colon carcinoma HCT116 cultures were treated with 10 % FBS for three ...
Background publications Silva, Hamamoto, Kunizaki et al.: "Enhanced methyltransferase activity of SMYD3 by the cleavage of its N-terminal region in human cancer cells." in: Oncogene, Vol. 27, Issue 19, pp. 2686-92, 2008 (PubMed).

Catalog No. ABIN406136
$289.00
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