3-hydroxyisobutyrate Dehydrogenase (HIBADH) (Middle Region) antibody

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Antigen
Synonyms NS5ATP1, 6430402H10Rik, AI265272, hibadh, zgc:66262
Epitope
Middle Region
(17), (17), (10), (4), (3), (2), (2), (1), (1), (1)
Reactivity
Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(76), (23), (23), (3), (1), (1), (1)
Host
Rabbit
(73), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(47), (33), (21), (12), (11), (3), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the middle region of human HIBADH
Blocking Peptide Blocking peptide for this product available: 3-hydroxyisobutyrate Dehydrogenase (HIBADH) (Middle Region) peptide (ABIN977973)
Sequence AKEVEKMGAV FMDAPVSGGV GAARSGNLTF MVGGVEDEFA AAQELLGCMG
Predicted Reactivity African clawed frog : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Chicken : 90 %, Zebrafish : 90 %
Characteristics This is a rabbit polyclonal antibody against HIBADH. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name HIBADH (HIBADH Antibody Abstract)
Background 3-hydroxyisobutyrate dehydrogenase (3-hydroxy-2-methylpropanoate:NAD(+) oxidoreductase, EC 1.1.1.31) is a dimeric mitochondrial enzyme that catalyzes the NAD(+)-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.3-hydroxyisobutyrate dehydrogenase (3-hydroxy-2-methylpropanoate:NAD(+) oxidoreductase, EC 1.1.1.31) is a dimeric mitochondrial enzyme that catalyzes the NAD(+)-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.
Molecular Weight 35 kDa
Gene ID 11112
NCBI Accession NM_152740, NP_689953
UniProt P31937
Research Area Metabolism, Amino Acids
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 336 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-3-hydroxyisobutyrate Dehydrogenase (HIBADH) (Middle Region) antibody (ABIN406311) WB Suggested Anti-HIBADH Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Background publications Hughes, Frutiger, Paquet et al.: "Human liver protein map: update 1993." in: Electrophoresis, Vol. 14, Issue 11, pp. 1216-22, 1994 (PubMed).

Catalog No. ABIN406311
$289.00
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