Non-SMC Condensin II Complex, Subunit H2 (NCAPH2) (N-Term) antibody

Details for Product No. ABIN406536
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Antigen
Synonyms MGC81656, CAPH2, si:dkey-202b22.2
Epitope
N-Term
(15), (2), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Human
(20), (3), (3), (1)
Host
Rabbit
(20)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(18), (8), (3), (3), (2), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN406536
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human NCAPH2
Sequence EYLYSLVYQA LDFISGKRRA KQLSSVQEDR ANGVASSGVP QEAENEFLSL
Predicted Reactivity Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Dog : 90 %, Horse : 90 %, Pig : 90 %
Characteristics This is a rabbit polyclonal antibody against NCAPH2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name NCAPH2
Background Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4, but they contain different sets of non-SMC subunits. NCAPH2 is 1 of 3 non-SMC subunits that define condensin II.Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 (MIM 605576) and SMC4 (MIM 605575), but they contain different sets of non-SMC subunits. NCAPH2 is 1 of 3 non-SMC subunits that define condensin II (Ono et al., 2003 [PubMed 14532007]).
Molecular Weight 68 kDa
Gene ID 29781
NCBI Accession NP_689512, NM_152299
UniProt Q6IBW4
Research Area Chromatin and Nuclear Signaling, Cell Cycle
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 605 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Non-SMC Condensin II Complex, Subunit H2 (NCAPH2) (N-Term) antibody WB Suggested Anti-NCAPH2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Hela cell lysate
Background publications Ono, Losada, Hirano et al.: "Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cells." in: Cell, Vol. 115, Issue 1, pp. 109-21, 2003 (PubMed).

Validation Images
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