heat shock 70kDa protein 9 (mortalin) (HSPA9), nuclear gene encoding mitochondrial protein antibody

Antigen: heat shock 70kDa protein 9 (mortalin) (HSPA9), nuclear gene encoding mitochondrial protein

Clonality: Monoclonal (4A1)

Host: Mouse

Reactivity: Human, Dog (Canine), Rat (Rattus), Monkey

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN410907

Additional Information

Gene ID: 3313

Immunogen: Protein expressed in 293T cell transfected with human HSPA9 expression vector

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 4A1

Molecular Weight: 73.7 KDa

Application Details

Application Notes: WB 1:2000, IF 1:100,

Concentration: 0.5-1.0 mg/ml (Lot dependent)

Purity: Purified

Purification: Purified from mouse ascites fluids by affinity chromatography

Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Restrictions: For Research Use only

Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA9 . Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA9 monoclonal antibody. IF Image Anti-HSPA9 mouse monoclonal antibody (ABIN410907) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9 (RC201397).

anti-heat shock 70kDa protein 9 (mortalin) (HSPA9), nuclear gene encoding mitochondrial protein antibody (Image 2)

Anti-HSPA9 mouse monoclonal antibody (ABIN410907) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9 (RC201397). Immunofluorescent staining of HT29 cells using anti-HSPA9 mouse monoclonal antibody (ABIN410907).