V-Akt Murine Thymoma Viral Oncogene Homolog 3 Protein Kinase B, Gamma, Transcript Variant 1 (AKT3) antibody

Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 3 Protein Kinase B, Gamma, Transcript Variant 1 (AKT3)

Clonality: Monoclonal (9H8)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Catalog no.: ABIN411251

Additional Information

Alternative name: v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) (AKT3), transcript variant 1

Gene ID: 10000

Immunogen: Recombinant fragment expressed in E.coli corresponding to amino acids 2-479 of human AKT3

Format: Purified

Isotype: IgG3  (Matching secondary antibodies)

Clone: 9H8

Molecular Weight: 55.6 kDa

Application Details

Application Notes: Western blot: 1:500, IHC 1,50, IF (1:100)

Concentration: 0.5-1.0 mg/ml (Lot dependent)

Purity: Purified

Purification: Purified from mouse ascites fluids by affinity chromatography

Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Research Area: Cancer

Restrictions: For Research Use only

Images

Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-AKT3 (ABIN411251) mouse monoclonal antibody. IF Image Anti-AKT3 mouse monoclonal antibody (ABIN411251) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY AKT3(RC221051)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3(RC221051) (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.

Anti-AKT3 mouse monoclonal antibody (ABIN411251) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY AKT3(RC221051) Immunofluorescent staining of HeLa cells using anti-AKT3 mouse monoclonal antibody (ABIN411251).