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INHBA ELISA Kit

INHBA Reactivity: Rat AA 311-426 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum
Catalog No. ABIN411256
  • Target See all INHBA ELISA Kits
    INHBA (Inhibin, beta A (INHBA))
    Binding Specificity
    AA 311-426
    Reactivity
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6-1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Activin A
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: CHO
    Immunogen sequence: G311-S426
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Predicted Reactivity
    Bovine,Monkey,Rabbit
    Sensitivity
    <12pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: CHO
    Immunogen sequence: G311-S426
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plate
    Pre-coated
    Protocol
    rat Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been precoated onto 96-well plates. Standards(CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Activin A amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat Activin A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat Activin A standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 97, Standard deviation: 4.9, CV(%): 5
    • Sample 2: n=16, Mean(pg/ml): 294, Standard deviation: 14.7, CV(%): 5
    • Sample 3: n=16, Mean(pg/ml): 459, Standard deviation: 23.9, CV(%): 5.2,
    • Sample 1: n=24, Mean(pg/ml): 103, Standard deviation: 8.0, CV(%): 7.8
    • Sample 2: n=24, Mean(pg/ml): 315, Standard deviation: 19.8, CV(%): 6.3
    • Sample 3: n=24, Mean(pg/ml): 512, Standard deviation: 24.6, CV(%): 4.8
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Zhou, Qu, Jin, Yang: "The extracts of Pacific oyster (Crassostrea gigas) alleviate ovarian functional disorders of female rats with exposure to bisphenol a through decreasing FSHR expression in ovarian tissues." in: African journal of traditional, complementary, and alternative medicines : AJTCAM, Vol. 11, Issue 5, pp. 1-7, (2015) (PubMed).

  • Target See all INHBA ELISA Kits
    INHBA (Inhibin, beta A (INHBA))
    Alternative Name
    INHBA (INHBA Products)
    Synonyms
    EDF ELISA Kit, FRP ELISA Kit, actba ELISA Kit, inhba ELISA Kit, inhbaa-001 ELISA Kit, edf ELISA Kit, frp ELISA Kit, INHB ELISA Kit, inhbal ELISA Kit, zgc:158348 ELISA Kit, inhibin beta A subunit ELISA Kit, inhibin beta-A ELISA Kit, inhibin, beta Aa ELISA Kit, inhibin beta A ELISA Kit, inhibin, beta Ab ELISA Kit, INHBA ELISA Kit, Inhba ELISA Kit, inhbaa ELISA Kit, inhba ELISA Kit, inhbab ELISA Kit
    Background

    Protein Function: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

    Background: Activin A is a homodimer of 14 kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.

    Synonyms: Inhibin beta A chain,Activin beta-A chain,Inhba,

    Full Gene Name: Inhibin beta A chain

    Cellular Localisation: Secreted.
    Gene ID
    29200
    UniProt
    P18331
    Pathways
    Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Autophagy
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