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Antibodies
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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody
| Antigen |
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| Synonyms |
PKBB, PRKBB, PKBBETA, RAC-BETA, AKT2, cb945, prkbb, v-akt2, pkbbeta, rac-beta |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (99), Mouse (Murine) (43), Rat (Rattus) (40), Cow (Bovine) (3), Monkey (3), Chicken (2), Dog (Canine) (2), Guinea Pig (2), Hamster (2), Pig (Porcine) (2), Rabbit (2), Sheep (Ovine) (2), Xenopus laevis (2), Primate (1)
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Application
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Western Blotting (WB) (87), Immunofluorescence (IF) (35), ELISA (31), Flow Cytometry (FACS) (25), Immunohistochemistry (IHC) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Enzyme Immunoassay (EIA) (19), Immunoprecipitation (IP) (9), Dot Blot (Dot) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN411406 |
| Quantity |
100 µg (0.25 mg/ml) |
| Price |
285.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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100 µg (0.25 mg/ml) affinity purified antibody. Reacts with human. Tested in ELISA and IHC.
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Alternative name
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Akt2
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Gene ID
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208
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Immunogen
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Synthetic peptide selected from the C-terminal region of human Akt2
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Isotype
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IgG (Matching secondary antibodies)
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Description
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AKT2 is a putative oncogenic protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
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Specificity
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The antibody detects a ~ 56 kDa band, corresponding to the expected molecular mass of Akt2 on immunoblots.
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Application Notes
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The antibody can be used for ELISA (0.25 µg/ml) and Western Blotting (0.5 – 2.5 µg/ml).
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Concentration
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0.25 mg/ml
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Purification
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Affinity Purified Antibody
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Buffer
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100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
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Storage
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Research Area
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Cardiovascular, Atherosclerosis, Signaling
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Restrictions
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For Research Use only
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Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2)", type "Antibodies"
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Hosts
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Rabbit (73), Mouse (36), Chicken (1)
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Reactivities
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Human (99), Mouse (Murine) (43), Rat (Rattus) (40), Cow (Bovine) (3), Monkey (3), Chicken (2), Dog (Canine) (2), Guinea Pig (2), Hamster (2), Pig (Porcine) (2), Rabbit (2), Sheep (Ovine) (2), Xenopus laevis (2), Primate (1)
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Applications
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Western Blotting (WB) (87), Immunofluorescence (IF) (35), ELISA (31), Flow Cytometry (FACS) (25), Immunohistochemistry (IHC) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Enzyme Immunoassay (EIA) (19), Immunoprecipitation (IP) (9), Dot Blot (Dot) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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PE (2), Agarose Beads (1), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pSer474 (10), C-Term (2), N-Term (2), Center (1), S474 (1), pS474 (1)
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