AKT3 antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN411410 |
| Quantity |
100 µg (0.25 mg/ml) (Variants) |
| Price |
285.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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100 µg (0.25 mg/ml) affinity purified antibody. Reacts with human (mouse). Tested in ELISA and WB.
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Gene ID
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10000
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Immunogen
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Synthetic peptide corresponding to amino acids surrounding T447 of human Akt3
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Isotype
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IgG (Matching secondary antibodies)
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Description
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AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1).
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Specificity
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The antibody detects a ~ 56 kDa band, corresponding to the expected molecular mass of Akt3 on immunoblots.
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Application Notes
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The antibody can be used for ELISA (0.25 µg/ml) and Western Blotting (2.5 – 5.0 µg/ml).
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Concentration
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0.25 mg/ml
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Purification
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Affinity Purified Antibody
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Buffer
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100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
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Storage
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Research Area
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Cancer
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Restrictions
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For Research Use only
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Alternatives for antigen "AKT3", type "Antibodies"
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Hosts
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Rabbit (31), Mouse (10), Goat (3), Chicken (1)
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Reactivities
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Human (41), Mouse (Murine) (16), Rat (Rattus) (16)
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Applications
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Western Blotting (WB) (26), ELISA (21), Immunofluorescence (IF) (21), Flow Cytometry (FACS) (19), Immunohistochemistry (IHC) (5), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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N-Term (1), S472 (1), T447 (1), internal (1)
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