Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) antibody

Antigen: Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2)

Synonyms: AMPK, AMPK2, PRKAA, A830082D05, AMPKalpha2, 2310008I11Rik, AMPKA2, prkaa1, PRKAA2, DKFZp468F2023

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN411417

Additional Information

Characteristics: 100 µg (0.25 mg/ml) affinity purified antibody. Reacts with human. Tested in ELISA and Western Blot.

Alternative name: AMPK2

Gene ID: 5563

Immunogen: Synthetic peptide selected from the center region of human AMPK2

Isotype: IgG  (Matching secondary antibodies)

Description: AMPK2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Specificity: The antibody detects a ~ 62 kDa band, corresponding to the expected molecular mass of AMPK2 on immunoblots.

Application Details

Application Notes: The antibody can be used for ELISA (0.25 µg/ml) and Western blotting (2.5 – 5.0 µg/ml).

Concentration: 0.25 mg/ml

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation

Buffer: 100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only