Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) antibody

Antigen: Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1)

Synonyms: AMPK, HAMPKb, MGC17785, AU021155, E430008F22, 1300015D22Rik, MGC82489, PRKAB1, DKFZp469J1020

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN411418

Additional Information

Characteristics: 100 µg (0.25 mg/ml) affinity purified antibody. Reacts with human/mouse. Tested in ELISA, Western Blot and IHC.

Alternative name: AMPKbeta

Gene ID: 5564

Immunogen: Synthetic peptide selected from the N-terminal region of human PRKAB1

Isotype: IgG  (Matching secondary antibodies)

Description: AMPK beta-1 chain is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

Specificity: The antibody detects a ~30 kDa band, corresponding to the expected molecular mass of AMPK β (PRKAB1) on immunoblots.

Application Details

Application Notes: The antibody can be used for ELISA (0.25 µg/ml), Western blotting (0.5 – 2.5 µg/ml) and Immunohistochemistry (2.5-5.0 µg/ml).

Concentration: 0.25 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only