Protein Kinase, AMP-Activated, gamma 3 Non-Catatlytic Subunit (PRKAG3) antibody

Antigen: Protein Kinase, AMP-Activated, gamma 3 Non-Catatlytic Subunit (PRKAG3)

Synonyms: Amkg3, Ampkg3, AMPKg3L, AMPKg3S, MGC52860, AMPKG3, PRKAG3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN411419

Additional Information

Characteristics: 100 µg (0.25 mg/ml) affinity purified antibody. Reacts with human/mouse. Tested in ELISA, Western Blot and IHC.

Alternative name: AMPKgamma

Gene ID: 5571

Immunogen: Synthetic peptide selected from the center of human PRKAG1

Isotype: IgG  (Matching secondary antibodies)

Description: AMPK gamma-1 chain is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK.

Specificity: The antibody detects a ~38 kDa band, corresponding to the expected molecular mass of AMPK γ (PRKAG1) on immunoblots.

Application Details

Application Notes: The antibody can be used for ELISA (0.25 µg/ml), Western blotting (0.5 – 2.5 µg/ml) and Immunohistochemistry (2.5-5.0 µg/ml).

Concentration: 0.25 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only