Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5) antibody

Antigen: Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5)

Synonyms: ASP, APG5, APG5L, hAPG5, APG5-LIKE, Apg5l, Atg5l, Paddy, C88337, AW319544, 2010107M05Rik, 3110067M24Rik, ATG5, apg5l, zgc:100934, DKFZp469P1819

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus), Pig (Porcine), Cow (Bovine)

Application: Western Blotting (WB)

Catalog no.: ABIN411423

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-Apg5/Atg5 polyclonal antibody. Detects 33 kDa Apg5 in Western blot analysis. A band at ~48 kDa can also be detected, presumble to be the Apg12-Apg5 complex. Reacts with human/mouse/rat/pig/bovine. Other applications have not been determined. Blocking peptide (Cat.# 3886BP-50) is also available.

Alternative name: Apg5/Atg5

Gene ID: 365601

Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 226 of rat Apg5

Isotype: IgG  (Matching secondary antibodies)

Description: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. An ubiquitin like conjugation system is involve in the formation of autophagosome where by Atg12 is covanletly bound to Apg5/ATG5. The conjugation is mediated by the ubiquitin-E1-like enzyme Atg7 and thE2-like enzyme Atg10.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~37 kDa Apg5/Atg5 from samples of human, mouse and rat origins. Reactivity to other species has not been tested.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only