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Antibodies
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anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) antibody
ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) antibody
| Antigen |
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| Synonyms |
atg7, D8, UBE2, UBA1B, UBE1L, MGC12713, MOP-4, UBE1L2, FLJ10808, FLJ23367, Uba7, 1300004C08Rik, Ube1l, Ube1l2, RGD1308324, UBA7, UBE1, UBA6 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Immunofluorescence (IF) (28), Western Blotting (WB) (19), Flow Cytometry (FACS) (18), Enzyme Immunoassay (EIA) (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (IHC) (5), ELISA (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN411424 |
| Quantity |
100 µg (0.5 mg/ml) |
| Price |
235.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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100 ug affinity purified rabbit anti-APG7/ATG7 polyclonal antibody. Reacts with human/mouse. Tested in Western blot. Other applications have not been determined.
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Alternative name
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APG7/ATG7
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Gene ID
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312647
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Immunogen
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Synthetic peptide near the N-terminus of rat APG7
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog (4). In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.
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Application Notes
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Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined.
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Concentration
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0.5 mg/ml
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Purification
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Affinity Purified Antibody
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Buffer
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0.5 mg/ml affinity purified rabbit anti-APG7/ATG7 in PBS containing 30% glycerol, 0.5 mg/ml BSA and 0.01% thimerosal.
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Storage
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Restrictions
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For Research Use only
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Alternatives for antigen "ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7)", type "Antibodies"
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Hosts
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Rabbit (39), Sheep (2), Mouse (1)
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Reactivities
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Human (36), Mouse (Murine) (26), Rat (Rattus) (20), Chicken (1)
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Applications
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Immunofluorescence (IF) (28), Western Blotting (WB) (19), Flow Cytometry (FACS) (18), Enzyme Immunoassay (EIA) (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (IHC) (5), ELISA (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (7), N-Term (4)
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Alternatives 
