ApoE4 antibody

Antigen: ApoE4

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN411428

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-human ApoE4 polyclonal antibody. Tested in Western blot. Other applications have not been determined. Recombinant human ApoE4 (Cat.# 4699-50) can be used as a positive control.

Gene ID: 178853

Immunogen: Recombinant human ApoE4

Isotype: IgG  (Matching secondary antibodies)

Description: ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It’s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. It also binds with the LDL receptor (apo B/E). Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. Individuals heterozygous for the ApoE4 allele are at higher risk of late-onset Alzheimer’s disease.

Application Details

Application Notes: Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human ApoE4 (Cat.# 4699-50) can be used as a positive control.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-human ApoE4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only