Actin, beta (ACTB) antibody

Antigen: Actin, beta (ACTB)

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN411429

Additional Information

Alternative name: Beta-Actin

Gene ID: 81822

Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 243 of rat β-actin

Isotype: IgG

Description: Actin is expressed in all eukaryotic cells and is the major component of the cytoskeleton. At least six types of actin are present in mammalian tissues and fall into three classes. Alpha actin expression is limited to various types of muscle and it regulates contactile potentials for the muscle cells, whereas beta and gamma actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility.

Characteristics: 100 ug affinity purified rabbit anti-beta-Actin polyclonal antibody. Detects mainly the 15 kDa cleaved C-terminal fragment of beta-Actin. Reacts with human, mouse and rat. Perform well in Western blotting or as loading control. Other applications have not been determined.

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Application Details

Application Notes: Western blotting (0.5-2 µg/ml). However, the optimal concentrations should be determined individually

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only

Publications

Product: Tewari, Kalkunte, Murray et al.: "The water channel aquaporin 1 is a novel molecular target of polychlorinated biphenyls for in utero anomalies." in: The Journal of biological chemistry, Vol. 284, Issue 22, pp. 15224-32, 2009 (PubMed).

Komagata, Nakajima, Takagi et al.: "Human CYP24 catalyzing the inactivation of calcitriol is post-transcriptionally regulated by miR-125b." in: Molecular pharmacology, Vol. 76, Issue 4, pp. 702-9, 2009 (PubMed).