Bcl2 Modifying Factor (BMF) antibody

Antigen: Bcl2 Modifying Factor (BMF)

Synonyms: FLJ00065, AW260063, AW411872, BMF

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411435

Additional Information

Characteristics: 100 ug affinity purified Rabbit anti-BMF Polyclonal antibody. Detects 28 kDa BMF from sample of human, mouse, and rat origins in Western blotting. Blocking peptide (Cat.# 3334RBP-50) is also available separately.

Alternative name: Bmf

Gene ID: 90427

Immunogen: Synthetic peptide surrounding amino acid 9 of human BMF

Isotype: IgG  (Matching secondary antibodies)

Description: Bmf (for Bcl-2-modifying factor) is a novel BH3-only protein recently identified in human and mouse. The BH3 domain in Bmf is required for both binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptosis program.

Specificity: The antibody recognizes ~28 kDa BMF in sample from human, mouse and rat origins. Reactivity to other species has not been tested. Blocking peptide (Cat.# 3334RBP-50) is available separately.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~28 kDa BMF in sample from human, mouse and rat origins. Reactivity to other species has not been tested.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-BMF polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Research Area: Signaling, Cytoskeleton, Apoptosis/Necrosis, Cancer

Restrictions: For Research Use only