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Antibodies
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anti-Complement Component 5a Receptor 1 (C5AR1) antibody
Complement Component 5a Receptor 1 (C5AR1) antibody
| Antigen |
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| Synonyms |
C5A, C5AR, C5R1, CD88, C5aR, C5r1, Cd88, D7Msu1, C5AR1, GPR77, C5ar1 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Flow Cytometry (FACS) (44), Immunofluorescence (IF) (21), Western Blotting (WB) (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunohistochemistry (Frozen Sections) (IHC (fro)) (11), ELISA (6), Enzyme Immunoassay (EIA) (5), Functional Studies (Func) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Neutralization (Neut) (1)
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| Catalog no. |
ABIN411444 |
| Quantity |
100 µg (0.5 mg/ml) |
| Price |
225.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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100 ug affinity purified rabbit anti-mouse C5a polyclonal antibody. Detects mouse C5a in Western Blot analysis. Other applications have not been determined. Recombinant mouse C5a (Cat.# 4895-20) can be used as a positive control.
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Alternative name
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C5a
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Gene ID
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15139
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Immunogen
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E. coli expressed mouse C5a
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Mouse complement C5a is a 77-amino acid peptide generated during complement activation from the alpha-chain of complement C5. Mouse C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
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Application Notes
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Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant mouse C5a (Cat.# 4895A-20) can be used as a positive control. Other applications have not been determined.
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Concentration
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0.5 mg/ml
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Purification
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Affinity Purified Antibody
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Buffer
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100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
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Storage
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Research Area
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Complement Factors
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Restrictions
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For Research Use only
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Alternatives for antigen "Complement Component 5a Receptor 1 (C5AR1)", type "Antibodies"
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Hosts
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Rabbit (29), Mouse (16), Rat (14), Chicken (1)
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Reactivities
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Mouse (Murine) (33), Human (26), Rat (Rattus) (21), Rabbit (3), Dog (Canine) (2), Cow (Bovine) (1)
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Applications
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Flow Cytometry (FACS) (44), Immunofluorescence (IF) (21), Western Blotting (WB) (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunohistochemistry (Frozen Sections) (IHC (fro)) (11), ELISA (6), Enzyme Immunoassay (EIA) (5), Functional Studies (Func) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Neutralization (Neut) (1)
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Conjugates
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PE (6), Biotin (3), FITC (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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Ab-338 (1), pSer338 (1)
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Alternatives 
