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Cathepsin D (CTSD) antibody

Antigen

Cathepsin D (CTSD)
Synonyms CPSD, CLN10, MGC2311, CD, CatD, catD, fb93e11, fj17b09, wu:fb93e11, wu:fj17b09, CTSD, LOC398557, Cathd, LOC100136761, DKFZp469J0315
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Application
Alternatives Western Blotting (WB)
Catalog no. ABIN411447
Quantity 100 µg  (0.5 mg/ml)
Price 225.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies back to top

Characteristics 100 ug affinity purified rabbit anti-Cathepsin D polyclonal antiboody. Detects the Procathepsin D (52 kDa) and also the active forms of cathepsin D (34 kDa). Reacts with human, mouse and rat. Tested in Western blot analysis. Blocking peptide (Cat.# 3191RBP-50) is available separately.
Alternative name Cathepsin D
Gene ID 13033
Immunogen Synthetic peptide surrounding amino acid 140 of mouse cathepsins D
Isotype IgG  (Matching secondary antibodies)
Description Cathepsin D is a normal lysosomal protease that is expressed in all cells. It is an aspartyl protease with a pH optimum in the range of 3-5, and contains two N-linked oligosaccharides. Cathepsin D is synthesized as an inactive 52 kDa pro-enzyme. Activation involves the proteolytic removal of the 43 amino acid profragment and an internal cleavage to generate the two-chain form made up of 34 and 14 kDa subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal that targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, Cathepsin D is abnormally processed and is secreted in its 52 kDa precursor form. Numerous clinical studies as well as in vitro evidence suggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer.

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Western blot analysis (0.5-4 µg/ml) and in immunoprecipitation. However, the optimal conditions should be determined individually. The purified antibody detects both the 52 kDa proform and the 34 kDa cleaved fragment of Cathepsin D.
Concentration 0.5 mg/ml
Purification Affinity Purified Antibody
Buffer 100 µg (0.5 mg/ml) affinity purified rabbit anti-cathepsin D polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Storage Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
Research Area Proteolysis / Ubiquitin, Extracellular Matrix, Proteases, Signaling
Restrictions For Research Use only