Dickkopf Homolog 2 (Xenopus Laevis) (DKK2) antibody

Antigen: Dickkopf Homolog 2 (Xenopus Laevis) (DKK2)

Synonyms: DKK-2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411465

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-DKK2. Reacts with human/mouse/rat. Detects ~57 kDa Dkk2 in Western blot analysis. Blocking peptide is available separately.

Alternative name: DKK2

Gene ID: 56811

Immunogen: Synthetic peptide surrounding amino acid 241 of mouse Dkk2

Isotype: IgG  (Matching secondary antibodies)

Description: Xenopus Dickkopf (Dkk)-1 was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of Dkk have been identified in mammals. Each Dkk molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of Dkk1 and Dkk2 inhibited Wnt-3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of Dkk-2, but not that of Dkk-1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl.

Specificity: The antibody recognizes 57 kDa Dkk2 in samples from human, mouse and rat origins. A ~55 kDa and ~28 kDa Dkk2 fragments can also be detected in rat kidney tissue lysate. Reactivity to other species has not been determined.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes 57 kDa Dkk2 in samples from human, mouse and rat origins. A ~55 kDa and ~28 kDa Dkk2 fragments can also be detected in rat kidney tissue lysate. Reactivity to other species has not been determined.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit Dkk2 polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Research Area: Stem Cells, Wnt

Restrictions: For Research Use only