Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) antibody

Antigen: Dickkopf Homolog 4 (Xenopus Laevis) (DKK4)

Synonyms: DKK-4, MGC129562, MGC129563

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411467

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-DKK4 polyclonal antibody. Reacts with human/mouse/rat. Detects ~40 Kda band in Western blot analysis. Other applications have not been determined. Blocking peptide (Cat.# 3894BP-50) is also available.

Alternative name: DKK4

Gene ID: 234130

Immunogen: Synthetic peptide surrounding amino acid 179 of mouse Dkk4

Isotype: IgG  (Matching secondary antibodies)

Description: Xenopus Dickkopf (Dkk) was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of Dkk have been identified in mammals. Each Dkk molecule contains two conserved cysteine-rich domains which are separated by a 50-55 amino acid linker region. Dkk4 is a secreted glycoprotein and has three immune-reactive sites at 15-17kDa, 30-32kDa and at 40kDa.

Specificity: The antibody detects ~40 kDa of Dkk4 in samples from human, mouse and rat origins. Reactivity to other species has not been determined.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~40 kDa of Dkk4 in samples from human, mouse and rat origins. Reactivity to other species has not been determined.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) antigen affinity purified rabbit anti-Dkk4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% Thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Research Area: Stem Cells, Wnt

Restrictions: For Research Use only