DR4 antibody

Antigen: DR4

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN411472

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-DR4 (also called Death Receptor 4, TRAIL-R1, TNFRSF10A, etc.) polyclonal antibody. Detects 55-65 kDa human DR4 protein in Western blotting. Reacts with human. Other species have not been tested. Blocking peptide (Cat.# 3061R-50) is available separately.

Gene ID: 8797

Immunogen: Synthetic peptide corresponding to residues surrounding amino acids 454 of human DR4

Isotype: IgG  (Matching secondary antibodies)

Description: Apoptosis can be induced by certain cytokines including TNF and Fas Ligand of the TNF super-family through their death domain (DD) containing receptors, TNFR1 and Fas. A member of the TNF receptor family was recently identified and designated DR4 (death receptor 4) and TRAIL-R1. The ligand for this novel death receptor has been identified and termed TRAIL. DR4 is expressed in most of the human tissues including spleen, peripheral blood leukocytes (PBLs), small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NFkB activation in many tissues and cells.

Specificity: The antibody detects a 57 kDa in samples from human origin. Jurkat cell lysate can be used as a positive control.

Application Details

Application Notes: Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 57 kDa in samples from human origin. Jurkat cell lysate can be used as a positive control.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-DR4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Research Area: Immunology, Innate Immunity, Cytokines, Apoptosis/Necrosis, Death Receptors, Signaling

Restrictions: For Research Use only