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Antibodies
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anti-Endonuclease G (ENDOG) antibody
Endonuclease G (ENDOG) antibody
| Antigen |
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| Synonyms |
FLJ27463, CG8862, DmelCG8862, ENDOG, Tb08.10J17.140, Tb08.10J17.90, TBC1D13, endog |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (21), Flow Cytometry (FACS) (20), Immunofluorescence (IF) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), ELISA (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN411479 |
| Quantity |
100 µg (0.5 mg/ml) |
| Price |
225.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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100 ug affinity purified Rabbit anti-EndoG Polyclonal antibody. Detects 35 kDa EndoG in samples of human/mouse/rat origins. Tested in Western blotting. Other applications have not been determined. Blocking peptide (Cat.# 3336RBP-50) is available separately.
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Alternative name
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EndoG
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Gene ID
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362100
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Immunogen
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Synthetic peptide surrounding amino acid 280 of rat EndoG
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Endonuclease G (EndoG) is a mitochondrion-specific nuclease that specifically activated by apoptotic stimuli and translocates to the nucleus during apoptosis. In nucleus, EndoG induces nucleosomal fragmentation of DNA independent of caspase and DFF/CAD.
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Specificity
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The antibody recognizes ~35 kDa EndoG of human, mouse, and rat origins. Reactivity to other species has not been tested.
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Application Notes
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Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~35 kDa EndoG of human, mouse, and rat origins. Reactivity to other species has not been tested.
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Concentration
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0.5 mg/ml
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Purification
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Affinity Purified Antibody
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Buffer
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100 µg (0.5 mg/ml) affinity purified rabbit anti-EndoG polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
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Storage
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Restrictions
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For Research Use only
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Alternatives for antigen "Endonuclease G (ENDOG)", type "Antibodies"
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Hosts
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Rabbit (33), Mouse (5)
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Reactivities
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Human (20), Mouse (Murine) (10), Rat (Rattus) (9), Dog (Canine) (1), Monkey (1)
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Applications
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Western Blotting (WB) (21), Flow Cytometry (FACS) (20), Immunofluorescence (IF) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), ELISA (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (1)
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Alternatives 
