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Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) antibody
| Antigen | Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) |
| Synonyms | NOS, INOS, NOS2A, HEP-NOS, iNOS, Nos-2, Nos2a, i-NOS, NOS-II, eNOS, Nos-3, ecNOS, 2310065A03Rik, iNos, cNOS, EC-NOS, NOSIII, NOS3, NOS2, nos2, ECNOS |
| Clonality | Polyclonal |
| Host |
 Alternatives Rabbit |
| Reactivity |
Alternatives Human, Mouse (Murine), Rat (Rattus) |
| Application |
Alternatives Western Blotting (WB)
|
| Catalog no. | ABIN411480 |
| Quantity | 100 µg (0.5 mg/ml) |
| Price | 225.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Characteristics | 100 ug affinity purified rabbit anti-eNOS polyclonal antibody. Reacts with human/mouse/rat/hamster/dog/cattle/sheep/guinea pig. Detects the 140 kDa full length and the ~100 kDa and 70 kDa eNOS fragments in Western blot analysis. Blockign peptide (Cat.# 3426RBP-50) is avaialble separately. |
| Alternative name | eNOS |
| Gene ID | 24600 |
| Immunogen | Synthetic peptide surrounding amino acid 602 of rat eNOS |
| Isotype | IgG (Matching secondary antibodies) |
| Description | ENOS (Endothelial nitric-oxide synthase) is an important enzyme in the cardiovascular system. eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli. In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation. |
| Specificity | The antibody recognizes 140 kDa eNOS of human, mouse, and rat origins. Reactivity to other species has not been tested. |
Application Details
| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes 140 kDa eNOS of human, mouse, and rat origins. Reactivity to other species has not been tested. |
| Concentration | 0.5 mg/ml |
| Purification | Affinity Purified Antibody |
| Buffer | 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal. |
| Storage | Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use. |
| Restrictions | For Research Use only |
Alternatives
Alternatives for antigen "Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3)", type "Antibodies"
| Hosts | Rabbit (47), Mouse (10) |
| Reactivities | Human (46), Rat (Rattus) (32), Mouse (Murine) (29), Cow (Bovine) (3), Pig (Porcine) (2), Dog (Canine) (1), Horse (Equine) (1), Monkey (1), Sheep (Ovine) (1) |
| Applications | Western Blotting (WB) (33), Immunofluorescence (IF) (28), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (IHC) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), ELISA (7), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), ELISA (Detection) (1), Flow Cytometry (FACS) (1), Immunoprecipitation (IP) (1) |
| Epitopes | pSer1177 (8), Ab-1176 (3), pSer1176 (3), Ab-1179 (1), Center (1), pSer632 (1) |
