Fas (TNF Receptor Superfamily, Member 6) (FAS) antibody

Antigen: Fas (TNF Receptor Superfamily, Member 6) (FAS)

Synonyms: APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6, FASL, CD178, CD95L, TNFSF6, APT1LG1, lpr, APO1, TNFR6, Tnfrsf6, AI196731, gld, Fas-L, Faslg, Tnfsf6, Fas-Ligand, Fasl, CD95-L, Apt1Lg1, FASLG, LOC574159, fasl, zgc:162027, FAS

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411481

Additional Information

Characteristics: 100 ug affinity purified rabbit polyclonal antibody. Detects 51 kDa glycosylated Fas, 35 kDa native Fas, and a 29 kDa cleaved Fas fragment in Western blot analysis. Reacts with human/mouse/rat. Other applications have not been determined. Blocking peptide (3070RBP-50) is available separately.

Alternative name: Fas/Apo1

Gene ID: 14102

Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 32 of mouse Fas

Isotype: IgG  (Matching secondary antibodies)

Description: Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1, TNFR2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic “death domain” that is responsible for transmitting signal for apoptosis.

Synonyms: APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6, FASL, CD178, CD95L, TNFSF6, APT1LG1, lpr, APO1, TNFR6, Tnfrsf6, AI196731, gld, Fas-L, Faslg, Tnfsf6, Fas-Ligand, Fasl, CD95-L, Apt1Lg1, FASLG, LOC574159, fasl, zgc:162027, FAS

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The purified antibody detects a 55 kDa of glycosylated Fas, a 35 kDa native form of Fas and a 29 kDa of cleaved Fas fragment on SDS-PAGE immunoblots.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only