Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1) antibody

Antigen: Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1)

Synonyms: FCNM, FCNB

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411486

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-Ficolin-1 polyclonal antibody. Reacts with human/mouse/rat. Tested in Western blot. Other applications have not been determined. Blocking peptide (Cat.# 3852BP-50) is also available.

Alternative name: Ficolin-1

Gene ID: 83517

Immunogen: Synthetic peptide surrounding amino acid 320 of rat Ficolin-1

Isotype: IgG  (Matching secondary antibodies)

Description: Ficolin-1 also known as M-Ficolin is a pattern recognition molecule of the lectin complement activation pathway. It is expressed mostly in monocytes, nuetrophils and in type II alveolar epithelial cells. Ficolin-1 is detected on the surface of circulating monocytes but is not present in the plasma. Ficolin-1 has a 28 aa signal sequence, an N-terminal collagen domain and a C-terminal fibrinogen-like (FBG) domain that contain a calcium binding site and a potential N-glycosylation site.

Specificity: The antibody recognizes ~33 kDa Ficolin-1 and a 37 kDa Ficolin-1 precursor in samples from human, mouse and rat origins. Reactivity to other species has not been tested.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~33 kDa Ficolin-1 and a 37 kDa Ficolin-1 precursor in samples from human, mouse and rat origins. Reactivity to other species has not been tested.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only