Ga15/16 antibody

Antigen: Ga15/16

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411491

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-Ga15/16 polyclonal antibody. Reacts with human/mouse/rat. Performs well in Western blotting. Blocking peptide (Cat.# 3250RBP-50 is also available separately.

Gene ID: 2769

Immunogen: Synthetic peptide surrounding amino acid 356of human G 15

Isotype: IgG  (Matching secondary antibodies)

Description: Gα16 is the only heterotrimeric G protein with a restricted expression pattern in hematopoietic cells. Differentiation of promyelocytic cells leads to decreased expression of Gα16. The G protein is known to couple a large number of G protein-coupled receptors to PLC-β and can lead to production of the secondary messengers diacylglycerol and inositol phosphates. Targeted deletion of Gα15, a mouse orthologue of human Gα16, causes reduced C5α receptor signaling. Gα16 serves as a marker, in addition to CD34, for hematopoietic progenitor cells.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml) and in immunohistochemistry (5-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects Gα15 in samples from human, mouse, rat, and rabbit origins. It also detects Gα16 from samples of human origin. Reactivity to other species has not been determined.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-Gα15 polyclonal antibody in PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only