Glucokinase (Hexokinase 4) (GCK) antibody

Antigen: Glucokinase (Hexokinase 4) (GCK)

Synonyms: GK, GLK, HK4, HHF3, HKIV, HXKP, MODY2, Gk, GCK, MGC166120, glk, hk4, hhf3, hkiv, hxkp, gck-A, mody2, glucokinase

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN411494

Additional Information

Characteristics: 100 µg (0.25 mg/ml) affinity purified antibody. Reacts with human and mouse. Tested in ELISA, WB and IHC.

Alternative name: GCK (Glucokinase)

Gene ID: 2645

Immunogen: Synthetic peptide selected from the C-terminal region of human GCK

Isotype: IgG  (Matching secondary antibodies)

Description: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of the gene for GCK results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in the gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), also called maturity onset diabetes of the young, type 2 (MODY2); mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

Specificity: The antibody detects a ~ 52 kDa band, corresponding to the expected molecular mass of GCK on immunoblots.

Application Details

Application Notes: The antibody can be used for ELISA (0.25 µg/ml), Western Blotting (0.5 – 2.5 µg/ml) and Immunohistochemistry (2.5 – 5.0 µg/ml).

Concentration: 0.25 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only