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GFP antibody

Antigen

GFP
Clonality Monoclonal (BV-F4)
Host
Alternatives

Mouse
Application
Alternatives Western Blotting (WB)
Catalog no. ABIN411495
Quantity 100 µg  (0.5 mg/ml)
Price 295.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies back to top

Characteristics 100 ug antigen affinity purified mouse anti-GFP monoclonal antibody (IgG1). Recognizes wild-type GFP and its derivatives such as EGFP/EBFP/ECFP/EYFP, etc. Performs well in Western blot analysis, immunoprecipitation, and immunohistochemistry. However, the optimum conditions should be determined individually.
Gene ID 110612126
Immunogen Recombinant EGFP expressed from E. col
Isotype IgG1  (Matching secondary antibodies)
Clone BV-F4
Description Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can also fuse with proteins of interest without interfering significantly with their assembly and function.
Specificity The antibody recognizes wild-type GFP and its derivatives such as EGFP, EBFP, ECFP, and EYFP, etc. Performs well in Western blot analysis (0.5 to 4 μ g/ml), immunoprecipitation, and immunohistochemistry. However, the optimum conditions should be determined individually.. Recombinant RFP (Cat.# 4997-100) can be used as a positive control.

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Western blotting (0.5-4 µg/ml), immunoprecipitation (2-8 µg/ml), immunofluorescence (10-20 µg/ml) and immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes wild-type GFP and its derivatives such as EGFP, EBFP, ECFP, and EYFP, etc. Performs well in Western blot analysis (0.5 to 4 µg/ml), immunoprecipitation, and immunohistochemistry. However, the optimum conditions should be determined individually.
Concentration 0.5 mg/ml
Purification Affinity Purified Antibody
Buffer 100 µg (0.5 mg/ml) antigen affinity purified mouse anti-GFP monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Storage Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
Research Area Tags/Labels
Restrictions For Research Use only