Hexokinase 2 (HK2) antibody

Antigen: Hexokinase 2 (HK2)

Synonyms: HKII, HXK2, DKFZp686M1669, AI642394, fi09d05, fi17h06, zgc:55926, wu:fi09d05, wu:fi17h06, wu:fi31b04, HK2, ARABIDOPSIS THALIANA HEXOKINASE 2, ATHXK2, F6F22.11, F6F22_11, HEXOKINASE 2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN411502

Additional Information

Characteristics: 100 µg (0.25 mg/ml) affinity purified antibody. Reacts with human. Tested in ELISA, Western Blot and IHC.

Alternative name: HK2 (Hexokinase II)

Gene ID: 3099

Immunogen: Synthetic peptide selected from the center region of human HK2

Isotype: IgG  (Matching secondary antibodies)

Description: In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.

Specificity: The antibody detects a ~ 102 kDa band, corresponding to the expected molecular mass of HK2 on immunoblots.

Application Details

Application Notes: The antibody can be used for ELISA (0.25 µg/ml), Western blotting (0.5 – 2.5 µg/ml) and Immunohistochemistry (2.5 – 5.0 µg/ml).

Concentration: 0.25 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only