Interleukin 12b (IL12B) antibody

Antigen: Interleukin 12b (IL12B)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN411514

Additional Information

Alternative name: IL-12p40

Gene ID: 3593

Immunogen: E. coli expressed recombinant human IL-12

Format: Liquid

Isotype: IgG

Description: Interleukin-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Recombinant Human IL-12 p40 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 306 amino acids fragment (23-328).

Characteristics: 100 ug affinity purified rabbit anti-human IL-12 polyclonal antibody. Detects mainly 40 kDa of IL-12p40 isoform, and in a lesser extent the 35 kDa of IL-12 p35 isoform. Tested in Western blot analysis. Recombinant human IL-12 (Cat.# 4732-10) can be used as a positive control.

Application Details

Application Notes: Western blot analysis (0.5-4 µg/ml). It detects mainly the 40 kDa of IL-12p40 isoform, and in a lesser extent the 35 kDa of IL-12p35 isoform. Recombinant human IL-12 (Cat.#:4732-10) can be used as a positive control. However, the optimal conditions should be determined individually.

Handling Advice: The antibody solution should be gently mixed before use.

Concentration: 0.5 mg/mL

Purification: Affinity purified

Buffer: phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% thimerosal.

Preservative: Thimerosal

Storage: -20 °C

Expiry Date: 12 months

Restrictions: For Research Use only