IL-18 antibody

Antigen: IL-18

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN411516

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-murine IL-18 polyclonal antibody. Tested in Western blotting/Immunoprecipitation/Neutralization.

Gene ID: 16173

Immunogen: Synthetic peptide of mouse IL-18

Isotype: IgG  (Matching secondary antibodies)

Description: IL-18 (IFN-gamma-inducing factor, IGIF) is a novel proinflammatory cytokine that augments natural killer (NK) activity in spleen cells. It also has other biological properties such as activation of NF-kB, stimulation of Fas ligand expression, induction of CC and CXC chemokines. IL-18 increases production of competent HIV. IL-18 is related to the IL-1 family. One of the IL-18 receptor chains is the IL-1 receptor-related protein, a member of the IL-1R family. Like IL-1β, IL-18 is first synthesized as a leaderless precursor requiring the IL-1β converting enzyme (ICE) for cleavage into an active molecule. IL-18 may play a role in tissue injury in inflammatory reactions.

Specificity: The antibody can be used for Western blot analysis (0.5-4 μ g/ml), immunoprecipitation (2-5 μ g/ml), and neutralization (2-5 μ g/ml). However, the optimal conditions should be determined individually.

Application Details

Application Notes: Western blot analysis (0.5-4 µg/ml), immunoprecipitation (2-5 µg/ml), and neutralization (2-5 µg/ml). However, the optimal conditions should be determined individually.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-mouse IL-18 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Research Area: Cytokines, Immunology, Virology, Inflammation, Cancer

Restrictions: For Research Use only

Publications

Publications: McCoy, Koizumi, Toma et al.: "Cytotoxins of the human pathogen Aeromonas hydrophila trigger, via the NLRP3 inflammasome, caspase-1 activation in macrophages." in: European journal of immunology, 2010 (PubMed).