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Insulin Induced Gene 1 (INSIG1) antibody

Antigen

Insulin Induced Gene 1 (INSIG1)
Synonyms CL6, CL-6, MGC1405, Insig-1, 1810013C12Rik, fb55a03, fi35b10, zgc:55439, wu:fb55a03, wu:fi35b10, insig1, MGC84597, INSIG1, MGC142724, cl-6, MGC147122
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus), Cow (Bovine), Hamster
Application
Alternatives Western Blotting (WB)
Catalog no. ABIN411520
Quantity 100 µg  (0.5 mg/ml)
Price 245.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies back to top

Characteristics 100 ug affinity purified rabbit anti-Insig1 polyclonal antibody. Reacts with human/mouse/rat/bovine/hamster. Tested in Western blot. Other applications have not been determined. Blocking peptide (Cat.# 3854BP-50) is also available separately.
Alternative name Insig1
Gene ID 64194
Immunogen Synthetic peptide surrounding amino acid 248 of rat Insig1
Isotype IgG  (Matching secondary antibodies)
Description Insulin-induced gene (InSig) localizes in the endoplasmic recticulum(ER) and is highly expressed in the liver and fibroblast cell lines. InSig1 and InSig2 play important roles in the regulation of cholesterol biosynthesis. Sterol induces InSig1 binding to the sterol-sensing domain of SREBP cleavage-activating protein (SCAP). Both InSig1 and Insig2 prevent the export of SCAP from the ER and thus, inhibit cholesterol synthesis by preventing the proteolytic cleavage of SREBPs by the Golgi enzymes.
Specificity The antibody recognizes ~30 kDa InSig-1 and its precursor (~50 kDa) in samples from human, mouse and rat origins. Reactivity to other species has not been tested.

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~30 kDa InSig-1 and its precursor (~50 kDa) in samples from human, mouse and rat origins. Reactivity to other species has not been tested.
Concentration 0.5 mg/ml
Purification Affinity Purified Antibody
Buffer 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Storage Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
Research Area Signaling
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies back to top

Alternatives for antigen "Insulin Induced Gene 1 (INSIG1)", type "Antibodies"
Hosts Rabbit (29)
Reactivities Human (23), Mouse (Murine) (20), Rat (Rattus) (19), Cow (Bovine) (3), Dog (Canine) (1), Guinea Pig (1), Pig (Porcine) (1), Rabbit (1), Sheep (Ovine) (1)
Applications Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (12), ELISA (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)