Insulin (INS) antibody
| Antigen |
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| Synonyms |
ILPR, IRDN, INS, MGC159719, ins1, xins, ins1-a, Insulin |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (182), Pig (Porcine) (91), Rat (Rattus) (58), Cow (Bovine) (45), Mouse (Murine) (45), Rabbit (29), Horse (Equine) (22), Dog (Canine) (9), Chicken (3), Guinea Pig (3), Sheep (Ovine) (3), Cat (Feline) (2)
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Application
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Immunofluorescence (IF) (77), Flow Cytometry (FACS) (71), Enzyme Immunoassay (EIA) (59), ELISA (51), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (41), Radioimmunoassay (RIA) (28), Immunohistochemistry (Frozen Sections) (IHC (fro)) (26), Western Blotting (WB) (25), Immunohistochemistry (IHC) (17), Immunoprecipitation (IP) (14), ELISA (Capture) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Functional Studies (Func) (4), Immunocytochemistry (ICC) (4), Immunoelectron Microscopy (IEM) (3)
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| Catalog no. |
ABIN411521 |
| Quantity |
100 µg (0.5 mg/ml) |
| Price |
225.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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100 ug affinity purified rabbit anti-human Insulin polyclonal antibody. Perform well in Western blot. Other applications have not been determined.
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Alternative name
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Insulin
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Gene ID
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3630
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Immunogen
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E. coli expressed recombinant human Insulin
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Insulin is important in stimulating the uptake of glucose from blood in the skeletal muscle and adipose tissue. It is secrered by pancreatic β cells through a glucose sensing pathway. Insulin also increases cell permeability to monosaccharides, amino acids and fatty acids. Insulin is first formed as a precursor molecule, preproinsulin which is then processed into proinsulin and finally to the mature insulin hormone. A mature form of insulin has an A chain and a B chain that are connnected by two dilsufide bonds. Defects in insulin are the cause of type-II diabetes mellitus.
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Specificity
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The antibody recognizes ~6 kDa Insulin in samples from human origins. Reactivity to other species has not been tested.
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Application Notes
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Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually.
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Concentration
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0.5 mg/ml
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Purification
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Affinity Purified Antibody
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Buffer
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100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
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Storage
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Research Area
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Neurology, Diabetes, Hormones
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Restrictions
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For Research Use only
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Alternatives for antigen "Insulin (INS)", type "Antibodies"
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Hosts
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Mouse (104), Rabbit (95), Guinea Pig (8), Sheep (4), Chicken (1)
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Reactivities
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Human (182), Pig (Porcine) (91), Rat (Rattus) (58), Cow (Bovine) (45), Mouse (Murine) (45), Rabbit (29), Horse (Equine) (22), Dog (Canine) (9), Chicken (3), Guinea Pig (3), Sheep (Ovine) (3), Cat (Feline) (2)
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Applications
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Immunofluorescence (IF) (77), Flow Cytometry (FACS) (71), Enzyme Immunoassay (EIA) (59), ELISA (51), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (41), Radioimmunoassay (RIA) (28), Immunohistochemistry (Frozen Sections) (IHC (fro)) (26), Western Blotting (WB) (25), Immunohistochemistry (IHC) (17), Immunoprecipitation (IP) (14), ELISA (Capture) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Functional Studies (Func) (4), Immunocytochemistry (ICC) (4), Immunoelectron Microscopy (IEM) (3)
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Conjugates
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Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), Biotin (6), PE,Cy5.5 (6), HRP (5), FITC (4), Alexa Flour 488 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy7 (3), RBITC (3)
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Epitopes
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beta chain (3), AA 57-87 (2), C-Term (2), Center (1)
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Alternatives 
