Leptin (LEP) antibody
| Antigen |
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| Synonyms |
OB, OBS, FLJ94114, ob, obese |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (95), Enzyme Immunoassay (EIA) (61), Immunofluorescence (IF) (61), Flow Cytometry (FACS) (55), ELISA (30), Immunohistochemistry (Frozen Sections) (IHC (fro)) (18), Radioimmunoassay (RIA) (15), Immunoprecipitation (IP) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Blocking Antibody (Inhibition) (4), ELISA (Detection) (3), Immunoelectron Microscopy (IEM) (3), ELISA (Capture) (2), Transfected cell culture (TCC) (2), Blocking Antibody (Inhibition) (1), Immunocytochemistry (ICC) (1), Immunohistochemistry (IHC) (1), Neutralization (Neut) (1)
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| Catalog no. |
ABIN411526 |
| Quantity |
100 µg (0.5 mg/ml) |
| Price |
225.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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100 ug affinity purified rabbit anti-rat leptin polyclonal antibody. Tested in Western blotting.
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Alternative name
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Leptin
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Gene ID
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25608
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Immunogen
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E. coli-expressed recombinant rat Leptin
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Leptin is a recently identified protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding leptin have been isolated from human, simian, mouse and rat cells. The expression of leptin mRNA has been shown to be restricted to adipose tissue. Leptin plays an important role in reproduction, immunological response and neuroendocrine signaling.
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Application Notes
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Western blot analysis (0.5-2 µg/ml). However, the optimal conditions should be determined individually. Recombinant rat Leptin (Cat.# 4368-02) can be used as a positive control. Other applications have not been tested.
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Concentration
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0.5 mg/ml
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Purification
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Affinity Purified Antibody
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Buffer
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100 µg (0.5 mg/ml) affinity purified rabbit anti-rat Leptin polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
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Storage
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Research Area
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Cardiovascular, Atherosclerosis, Hormones
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Restrictions
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For Research Use only
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Alternatives for antigen "Leptin (LEP)", type "Antibodies"
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Hosts
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Rabbit (131), Mouse (36), Goat (6), Sheep (2)
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Reactivities
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Human (104), Mouse (Murine) (92), Rat (Rattus) (79), Pig (Porcine) (35), Cow (Bovine) (19), Dog (Canine) (18), Sheep (Ovine) (17), Horse (Equine) (15), Rabbit (2), Chicken (1)
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Applications
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Western Blotting (WB) (95), Enzyme Immunoassay (EIA) (61), Immunofluorescence (IF) (61), Flow Cytometry (FACS) (55), ELISA (30), Immunohistochemistry (Frozen Sections) (IHC (fro)) (18), Radioimmunoassay (RIA) (15), Immunoprecipitation (IP) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Blocking Antibody (Inhibition) (4), ELISA (Detection) (3), Immunoelectron Microscopy (IEM) (3), ELISA (Capture) (2), Transfected cell culture (TCC) (2), Blocking Antibody (Inhibition) (1), Immunocytochemistry (ICC) (1), Immunohistochemistry (IHC) (1), Neutralization (Neut) (1)
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Conjugates
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Biotin (15), Alexa Flour 488 (3), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), HRP (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), RBITC (3)
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Epitopes
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C-Term (3), 92-145 cyclized (2), AA 1-24 (2), amino terminal residues 1-24 (2), mouse (1), rat (1), residues 131-145 (1)
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Alternatives 
